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Minusheet perfusion culture system
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Minusheet perfusion culture system : ウィキペディア英語版
Minusheet perfusion culture system

Minusheet perfusion culture system is used for advanced cell culture experiments in combination with adherent cells and to generate specialized tissues in combination with selected biomaterials, special tissue carriers and compatible perfusion culture containers.
The technical development of the Minusheet perfusion culture system was driven by the idea to create under in vitro conditions an environment resembling as near as possible the situation of specialized tissues found within the organism. Basis of this invention is therefore individually selected biomaterials for optimal cell adhesion mounted in Minusheet tissue carriers. Moreover, to always offer fresh nutrition including respiratory gas and to simulate a tissue-specific fluid environment, the tissue carriers can be inserted into compatible perfusion culture containers. As a result, a variety of publications illustrates that tissues generated by this innovative approach exhibit an excellent and stable quality. Thus, on the one hand the system provides a highly adaptable basis for the culture of adherent cells and the generation of specialized tissues. On the other hand the Minusheet perfusion culture system is bridging a methodical gap between the conventional static 24 well culture plate and modern perfusion culture technology.
==Crucial generation of specialized tissues==
Specialized tissues in culture are urgently needed in regenerative medicine, tissue engineering, nanotechnology, biomaterial research and advanced toxicity testing of newly developed pharmaceuticals. However, it is often observed that raised tissues do not exhibit expected functional features. Instead dedifferentiation is observed (). These cell biological alterations arise after isolation of cells and proceed during static culture in a dish due to suboptimal fluid environment and minor adhesion on biomaterials. Further uncontrolled supply with nutrition and respiratory gas, an overshoot of metabolites and paracrine factors or missing rheological stress can increase the degree of dedifferentiation. In consequence, regarding an optimal generation of specialized tissues a powerful strategy has to exclude as much as possible harmful parameters, while factors supporting the process of tissue development must be intensified ().

抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
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